Photoautotrophic microclonal propagation of raspberry (Rubus idaeus L.) variety Delniwa

Autorzy

• Vyacheslav Matskevych Bila Tserkva National Agrarian University, Department of Forestry, Bila Tserkva, Ukraine
• Vasyl Yukhnovskyi National University of Life and Environmental Sciences of Ukraine, Forest Restoration and Meliorations Department, Henerala Rodymtseva 19, 03041 Kyiv, Ukraine,
  e-mail: yukhnov@ukr.net
• Larysa Filipova Sumy National Agrarian University, Department of Biotechnology and Phytopharmacology, Sumy, Ukraine
• Natalia Kravchenko Sumy National Agrarian University, Department of Biotechnology and Phytopharmacology, Sumy, Ukraine
• Olga Tupchii National University of Life and Environmental Sciences of Ukraine, Department of Forests Restoration and Meliorations,
  Kyiv, Ukraine
• Yurii Matskevych Bila Tserkva National Agrarian University, Department of Agriculture, Agrochemistry and Soil Science, Bila Tserkva,
  Ukraine

Abstract

The purpose of the study is to optimize the trophic and hormonal determination of raspberry reproduction in vitro and post-aseptic adaptation ex vitro. The research was carried out on the remontant raspberry variety Delniwa of Polish selection. Laboratory studies were carried out in the conditions of the Tevitta TM laboratory (Ltd “Agrofirm Blagodatne,” Cherkasy region), and experiments were laid in four stages: introduction into aseptic culture, multiplication, induction of rhizogenesis, and post-aseptic adaptation, including photoautotrophic grafting.
A medium for obtaining an aseptic culture and a media for multiplication have been selected. The rate of regeneration of explants of different origins, and the percentage of regenerants from them, was compared.
The dynamics of the influence of cytokinin concentrations during long-term reproduction in vitro were revealed. Cultivation on a medium with a high content of benzylaminopurine and kinetin showed the effect of accumulation of phytotoxicity. The expediency of growing graft donors for grafting at low kinetin concentrations to improve the induction of rhizogenesis is substantiated. Regenerants from media with a predominance of auxins over cytokinins had a higher regeneration potential.
According to indicators of suitability for survival, perlite substrates prevailed over peat ones; however, regenerants of a smaller height were formed on them. The symptoms and harmful effects of excessive watering on plants have been established. A biomethod of insect control using insectivorous plants of the genus Drosera has been developed.
The rate of rhizogenesis in bioreactor conditions with humidified and carbon dioxide-enriched air significantly exceeded root formation in conventional moist chambers. The duration of growing an adapted and well-rooted seedling in a bioreactor was 21 days, compared to 30 or more days in the control version of the experiment. The plants did not lose turgor when planted in closed soil. Adapted regenerants are successfully used as a planting material and can be used in photoautotrophic microclonal propagation as donors of cuttings. Under such conditions, the regeneration efficiency is maintained for five cutting generations.

Keywords

• autotrophic nutrition
• heterotrophic nutrition
• biomethod
• in vitro
• raspberry
• regenerant
• nursery
• explant