Deciduous stump inoculation with Pleurotus ostreatus as a biological method of armillaria root rot control
Field experiment with application of biological preparation of Pleurotus ostreatus (in form of beech sawdust with growing mycelium) on deciduous tree stumps was performed in two mixed beech-oak-Scots pine/Norway spruce stands with single birch admixture. 16 and 24 months after the treatment beech, oak and birch stumps (one of each species) were rooted out and cut on 10-cm sections. Samples of sections wood were than incubated on malt-agar medium to check the presence of P. ostreatus mycelium. The fastest colonization was found for beech stumps – 16 months after treatment P. ostreatus was present on all wood samples and up to 20 cm from the stump surface, were the preparation was initially applied. Birch and especially oak wood was colonized much slower – on most samples fungus mycelium was found no more than 10 cm from the stump surface and on some was not present at all.
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