In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis

Autorzy

• Oksana Chornobrov National University of Life and Environmental Sciences of Ukraine, Boyarka Forestry Research Station, Plant Biotechnology Research Laboratory, Lisodoslidna 12, Boyarka 08150, Kyiv-Svyatoshyn distrist, Ukraine
• Svitlana Bilous National University of Life and Environmental Sciences of Ukraine, Botany, Dendrology and Forest Tree Breeding Department, General Rodimtsev 19, Kyiv 03041, Ukraine, e-mail: forest_biotech@nubip.edu.ua
  
  Institute for Evolutionary Ecology NAS Ukraine, Department of Dendrology and Park Learning, Lebedeva 37, Kyiv, 03143, Ukraine

Abstract

Plants of Schlumbergera truncata (Haw.) Moran were obtained by indirect morphogenesis from the segment section of shoots in vitro, they were multiplied and rooted. Also were determined the effect of the lighting regime, the composition of the nutrient medium on the consistency and frequency of callus formation. The studies were conducted during 2016–2018. The mode of effective sterilization (more than 90%) of S. truncata plant explants using 0.1% HgCl₂ for 7–8 min was established. Optimal conditions for the induction of callus formation in stem node segments of S. truncata plants (rate more than 90% and significant growth) were created on MS (Murashige and Skoog 1962) nutrient medium supplemented with 1.0 mg/l BAP (6-benzylaminopurine) and 0.3 mg/l NAA (1-naphthylacetic acid) under conditions of placement on the nutrient medium and doing a significant number of cuts on the explants. The light intensity of 2.0–3.0 klx, obtained by a callus of dense consistency of dark green pigmentation, when using the thermostat condition without illumination, the callus had loose consistency, dark yellow pigmentation. It is established that the influence of the lighting regime and the composition of the nutrient medium on the frequency of callus formation is statistically significant. The largest number of shoots was obtained on the MS medium with the addition of 2.0 mg/l of BA. At the same times, shoot proliferation and root induction in such numbers were observed on MS culture medium with the addition of 0.5 mg/l BA and 0.5 mg/l kinetin (multiplication factor – 8.8±0.6 per 60-day cultivation cycle).

Keywords

• Christmas cactus
• nutrient medium
• callus
• shoot proliferation
• microclonal propagation
• explant