Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia

Autorzy

  • Annisa Universitas Padjadjaran, Faculty of Mathematics and Natural Sciences, Department of Biology
    Indonesia
  • Rini Hafzari Universitas Padjadjaran, Faculty of Mathematics and Natural Sciences, Department of Biology
    Indonesia
  • Tia Setiawati Universitas Padjadjaran, Faculty of Mathematics and Natural Sciences, Department of Biology
    Indonesia
  • Budi Irawan Universitas Padjadjaran, Faculty of Mathematics and Natural Sciences, Department of Biology
    Indonesia
  • Joko Kusmoro Universitas Padjadjaran, Faculty of Mathematics and Natural Sciences, Department of Biology
    Indonesia

Abstract

Conservation of bamboos for future exploitation as fuel, fibre and as an ingredient for cosmetics depends on knowledge of its natural genetic variation. The study of molecular genetic diversity in bamboos will provide important information for its conservation. This article reports on the genetic diversity in 25 species representing five genera of bamboos found in Indonesia using Random Amplified Polymorphic DNA (RAPD) molecular markers. Out of 40 primers, 24 primers produced 1107 total bands and 86.21% of polymorphic bands across the 25 species. Sixteen bands were uniquely found in one species only and their presence or absence helped to define nine bamboo species. RAPD band sizes ranged from 162 to 2247 base pairs. A dendrogram based on the similarity coefficient of Dice divided the bamboo species into three big clusters. In conclusion, RAPD can capture the diversity among five different bamboo genera and has a great potential to be used in the study of genetic diversity in Indonesian bamboos.

DOI 10.2478/ffp-2019-0025
Source Folia Forestalia Polonica, Series A – Forestry
Print ISSN 0071-6677
Online ISSN
2199-5907
Type of article
original article
Original title
Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia
Publisher The Committee on Forestry Sciences and Wood Technology of the Polish Academy of Sciences and the Forest Research Institute in Sekocin Stary
Date 30/12/2019

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