First records of Phytophthora spp. based on DNA analysis in Lithuania

Autorzy

• Adomas Vitas Vytautas Magnus University, Faculty of Nature Sciences, Environmental Research Centre
  Ž.E. Žilibero 2, LT-46324 Kaunas, Lithuania
  e-mail: a.vitas@gmf.vdu.lt
• Tomasz Oszako Forest Research Institute, Department of Forest Protection, Sękocin Stary, Braci Leśnej 3, 05-090 Raszyn, Poland
• Justyna A. Nowakowska Forest Research Institute, Department of Silviculture and Genetics, Sękocin Stary, Braci Leśnej 3, 05-090 Raszyn, Poland
• Katarzyna Sikora Forest Research Institute, Department of Forest Protection, Sękocin Stary, Braci Leśnej 3, 05-090 Raszyn, Poland
• Antanina Stankevičienė Vytautas Magnus University, Kaunas Botanical Garden
  Ž.E. Žilibero 6, LT-46324 Kaunas, Lithuania

Abstract

The assessment of alien invasive species of Phytophthora genus causing serious forest tree species diseases was carried out in Lithuania. The presence of Phytophthora DNA was recorded for the first time using real-time PCR analysis on 23 DNA samples. The sampling includes wood from diseased trees, leaves from shrubs, leaves baited in water, and soil samples taken around diseased plants. Extracted DNA from soil and plant tissues was tested for the presence of Phytophthora. All analysed samples were positively recognized by Phytophthora-specific probe during real-time PCR which proves the presence of pathogens in environmental samples.

Keywords

• alien invasive species
• environmental samples
• Phytophthora specific DNA probes
• baiting
• qPCR
• oomycetes