Genetical variation of polish napiwodzko-ramucka and spalska scots pine provenances determined by mitochondrial dna analyses
One specific pair of STS primers amplifying the B/C intron of mitochondrial nad1 gene was used to determine the genetic diversity level in fourteen different stands of Scots pine in Poland. The studied stands were situated in seed region of napiwodzko-ramucka Scots pine provenance (no 205) and in seed region of spalska Scots pine provenance (no 601). For each region, the genetic diversity level and the genetic phylogeny were examined in order to check the exactness of the border lines previously defined for 205 and 601 seed region. In both regions, 205 and 601, four different alleles were detected, i.e. haplotypes „a”, „b”, „c” and „d” with different frequencies across stands. The most frequent haplotype „c” (mean frequency of 0.481) was uniformly distributed among stands. Haplotypes „a” and „d” were present with mean frequencies 0.392 and 0.144 respectively. The presence of the rare haplotype „b” (mean frequency of 0.017) was mainly observed in spalska pine region (601). Our results confirm the current rules of transfer of the forest reproductive material from the regions 106, 205 and 401 to the region 451 and from the stands of the region 601 to the stands of the region 651. Few changes in current directives may be proposed concerning some stands in the seed regions 106, 205, 251, 401, 453, 601, 603 and 604. A relatively high degree of differentiation (overall GST about 62.5%) characterized all studied pines suggesting large gene flow among the studied stands. Our founding may be useful for the further verification of Scots pine reproductive material transfer rules in Poland.
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