Micropropagation of european larch (Larix decidua Mill.) via somatic embryogenesis
The somatic embryogenesis has been used for propagation of European larch (Larix decidua Mill.). Somatic embryogenesis in combination with cryopreservation is very effective in preserving selected genotypes and mass (clonal) propagation. In this study, the main problem with larch somatic embryogenesis is to initiate the embryonal tissue. Immature zygotic embryos at precotyledonary stage with endosperm (megagametophyte) have been used and embryogenic callus frequency 8.6–35.7% have been obtained, as well as with cotyledonary zygotic embryos (8.6%). Development of mature somatic embryos at cotyledonary stage was observed after 4 weeks culture of embryogenic callus on basal medium (MSG) containing 20–60 µM ABA with 1µM IBA (dependent on genotype). Three generations of embryogenic callus and plantlets were obtained. Germination and development of plantles indicate possible use of this method during production of improved plant material in forestry.
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